Coding

Part:BBa_K1961007:Design

Designed by: Yi-Ting Lin   Group: iGEM16_HSiTAIWAN   (2016-10-12)


CYP1A2, an enzyme of cytochrome P450 protein family that metabolizes toxin in liver


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1400
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 320
    Illegal NgoMIV site found at 407
    Illegal AgeI site found at 390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1120


Design Notes

but we do some chang to remove the restriction site.


Source

We use humen liver gene cyp1a2(genebank:NM_000761)

References